Developing and improving omics technologies for single cells

Although considerable progress has been made in recent years, single-cell NGS technologies are still struggling with two major technical challenges: (1) homogeneous and representative amplification of genomic DNA and/or mRNA in amounts sufficient for downstream applications and (2) reliable identification of errors introduced into single-cell-derived nucleic acids by amplification. The Fraunhofer ITEM scientists have addressed both needs by developing approaches generating sufficient quantities of high-quality amplified material allowing for test repetitions and biobanking of amplified samples and, moreover, implementing barcoding strategies in the WGA approach comprising the elimination of sequence errors originating from WGA or sequencing.

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