Advanced preclinical PDX models

We optimize patient-derived xenograft (PDX) models for representative preclinical testing

© Photo Fraunhofer ITEM

Our commitment is to develop advanced preclinical PDX models to enable improved prediction of the pharmaceutical efficacy and adverse effects of drugs in vivo.

Preclinical animal models only partially represent the patient situation. Main disadvantages include the species barrier, the lack of immune cells in xenograft models, and the use of aggressive cell lines. Fraunhofer ITEM in Regensburg is developing optimized PDX models allowing more representative preclinical drug testing. Our advanced models are based on patient-derived metastatic precursor cells (disseminated tumor cells, DTCs) or circulating tumor cells (CTCs). In addition, we concomitantly generate a human immune system in these mice, which infiltrates the human tumors and develops phenotypes (e.g. tumor-associated macrophages) that have been described in patient samples. This allows both the tumor development and the dissemination of cancer cells into different organs to be followed in the presence of human immune cells. Our services include development of individualized preclinical in-vivo models to test in particular immunomodulatory drugs on target cells of systemic disease.

 

 

Our services and expertise

Generation of double-humanized mice:
Establishment of a human immune system in mice, patient-derived xenografts based on DTCs, CTCs, or primary tumor cells, quantification of tumor cell dissemination in organs

Characterization of tumor/immune cells:
Immunohistochemistry services, fluorescence-activated cell sorting of subpopulations, genomic and transcriptomic characterization of subpopulations

Your benefits

Individualized development of preclinical models based on the cells of interest

Customized assays developed for the mode of action of your drug

Access to a broad range of clinical samples

As a non-university research institution we can be your partner in applying for public funding

ITEM-R is DIN ISO 9001:2015 certified