Fraunhofer Institute for Toxicology and Experimental Medicine
Fraunhofer Institute for Toxicology and Experimental Medicine

Metabolomics Research

Targeted metabolomics by LC-MS/MS

The metabolome comprises the entirety of all small molecules found in the different compart­ments of an organism.
© Fraunhofer ITEM, Ralf Mohr
The metabolome comprises the entirety of all small molecules found in the different compart­ments of an organism.
Fraunhofer ITEM is a certified laboratory of Biocrates

We offer standardised and high-throughput quantitative metabolome analysis by LC-MS/MS and NMR spectroscopy. 

 

We are a certified laboratory of Biocrates®, offering standardised laboratory and kit workflow procedures. The kit-based approach presents the following advantages:

  • High analyte coverage by targeting 1019 metabolites from 39 biochemical substance classes per sample
  • High sample throughput, allowing the analysis of more than 600 samples within 2 weeks
  • Applicability to various matrices such as plasma, serum, urine, faeces and tissue samples from different species such as rat, mouse, human etc.
  • Standardised data processing, with data delivery in an editable Excel format facilitating transfer for data evaluation

Targeted metabolomics by NMR spectroscopy

We offer standardised NMR based metabolomics by applying Bruker IVDr (B.I.) Analysis packages.

 

Advantages of this approach are:

  • Fully quantitative determination of few hundred metabolites from several biochemical substance classes using:
    • B.I.Quant-PS - Quantification Engine Human Plasma & Serum
    • B.I.LISA - Lipoprotein Subclass Analysis for Human Plasma & Serum
    • B.I.Quant-UR ex - Human Urine Extended Quantification Method
  • Applicability to various body fluids such as plasma, serum, urine, CSF and methanol extracts of tissues etc. 
  • Non-destructive sample analysis with high reproducibility

 

The number of metabolites in any organism is vast and their entirety cannot be captured by a single analytical method.
© Fraunhofer ITEM, Ralf Mohr
The number of metabolites in any organism is vast and their entirety cannot be captured by a single analytical method.

Metabolomics Research at Fraunhofer ITEM - Contact us!

Get in touch with the project team

Thank you for contacting us. We have received your message and will get back to you as soon as possible. 

 

Best regards,

Your Metabolomics team at Fraunhofer ITEM 

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Thank you for contacting us. Unfortunately, something went wrong. Your message was not forwarded to us. Please try again on the webpage https://www.item.fraunhofer.de/en/services-expertise/chemical-safety-assessment/method-development-and-customized-analyses/targeted-metabolomics.html. We apologize for the inconvenience.

 

Thank you and best regards,

Your Metabolomics team at Fraunhofer ITEM 

Your contact for Bio- and Environmental Analytics

Sven Schuchardt

Contact Press / Media

Dr. Sven Schuchardt

Head of Department of Bio and Environmental Analytics

Phone +49 511 5350-218

Recent Publications

  • Shahrokny, P., Maison, N., Riemann; L., Ehrmann, M., DeLuca, D., Schuchardt, S.,Thiele, D., Weckmann, M., Dittrich, A. M., Schaub, B., Brinkmann, F., Hansen, G., Kopp, M. V., von Mutius, E., Rabe, K. F., Bahmer, T., Hohlfeld, J. M., Grychtol, R., Holz, O. and The ALLIANCE Study Group (2024): Increased breath naphthalene in children with asthma and wheeze of the All Age Asthma Cohort (ALLIANCE). Journal of Breath Research, 18:016003. https://iopscience.iop.org/article/10.1088/1752-7163/acf23e
  • Ringseis, R., Marschall, M.J.M., Grundmann, S.M., Schuchardt, S., Most, E., Gessner, D.K., Wen, G., Eder, K. (2023): Effect of Hermetia illucens Fat, Compared with That of Soybean Oil and Palm Oil, on Hepatic Lipid Metabolism and Plasma Metabolome in Healthy Rats. Animals, 13:3356. https://www.mdpi.com/2076-2615/13/21/3356
  • Dutsch, A., Uhlig, C., Bock, M., Graesser, C., Schuchardt, S., Uhlig, S., Schunkert, H., Joner, M., Holdenrieder, S., & Lechner, K. (2023): Multi-Omic Candidate Screening for Markers of Severe Clinical Courses of COVID-19. Journal of Clinical Medicine, 12:6225. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573369/
  • Starke, S., Harris, D.M.M., Zimmermann, J., Schuchardt, S., Oumari, M., Frank, D., Bang, C., Rosenstiel, P., Schreiber, S., Frey, N., Franke, A., Aden, K., Waschina, S. (2023):  Amino acid auxotrophies in human gut bacteria are linked to higher microbiome diversity and long-term stability. The ISME Journal. https://www.nature.com/articles/s41396-023-01537-3
  • Seeliger, B., Carleo, A.., Wendel-Garcia,  P.D., Fuge, J., Montes-Warboys, A., Schuchardt, S., Molina-Molina, M.,  Prasse, A. (2022): Changes in serum metabolomics in idiopathic pulmonary fibrosis and effect of approved antifibrotic medication. Frontiers in Pharmacology. 13.  https://www.frontiersin.org/articles/10.3389/fphar.2022.837680/full  
  • Diboun, I., Cyprian, F.S., Anwardeen, N.R., Yassine, H.M., Elrayess, M.A., Rahmoon, S.M., Sayed, S.K., Schuchardt, S., Khatib, M., Bansal, D., Farag, E.A.B.A., Emara, M.M., Abdallah, A.M. (2022): Identification of prognostic metabolomic biomarkers at the interface of mortality and morbidity in pre-existing TB cases infected with SARS-CoV-2. Frontier in Cellular and Infection Microbiology. 12:929689. https://www.frontiersin.org/articles/10.3389/fcimb.2022.929689/full
  • Elrayess, M.A., Cyprian, F.S., Abdallah, A.M., Emara, M.M., Diboun, I., Anwardeen, N., Schuchardt, S., Yassine, H.M.(2022): Metabolic signatures of type 2 diabetes mellitus and hypertension in COVID-19 patients with different disease severity. In: Front Med (Lausanne).8:788687. https://www.frontiersin.org/articles/10.3389/fmed.2021.788687/full
  • Holz, O., van Vorstenbosch, R., Guenther, F., Schuchardt, S., Trinkmann, F., von Schooten, F.J., Smolinska, A., Hohlfeld, J.M. (2022): Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge. In: Journal of Breath Research. 16 (3), 037102. https://pubmed.ncbi.nlm.nih.gov/35366648/
  • Chen, F., Elgaher, W.A.M., Winterhoff, M., Büssow, K., Waqas, F. H., Graner, E. Pires-Afonso, Y., Casares Perez, L., de la Vega, L., Sahini, N., Czichon, L., Zobl, W., Zillinger, T., Shehata, M., Pleschka, S., Bähre, H., Falk, C., Michelucci, A., Schuchardt, S., Blankenfeldt, W., Hirsch, A. K. H., Pessler, F. (2022): Citraconate inhibits ACOD1 (IRG1) catalysis, reduces interferon responses and oxidative stress, and modulates inflammation and cell metabolism. In: Nature Metabolism. 4:534. https://www.nature.com/articles/s42255-022-00577-x
  • Taleb, S., Yassine, H.M., Benslimane, F.M., Smatti, M.K., Schuchardt, S., Albagha, O., Al-Thani, A.A., Ait Hssain, A., Diboun, I., Elrayess, M.A. (2021): Predictive biomarkers of intensive care unit and mechanical ventilation duration in critically-Ill coronavirus disease 2019 Patients. In: Front Med (Lausanne).8:733657. https://www.frontiersin.org/articles/10.3389/fmed.2021.733657/full
  • Luo, Y., Möhn, N., Al-Mekhlafi, A., Schuchardt, S., Skripuletz, T., Sühs, W., et al. (2020): Targeted metabolomic profiling of cerebrospinal fluid from patients with progressive multifocal leukoencephalopathy. In: PLoS ONE. 15(11): e0242321. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242321