Fraunhofer Institute for Toxicology and Experimental MedicineUpstream: Mammalian cell culture
Services
- Screening of cell clones and cell lines for production feasibility parameters (stability, productivity, glycosylation patterns)
- Cell line adaptation from adherent to suspension culture and from serum-free to protein-free media
- Development of batch, fed-batch, and perfusion cultivation regimes
- Optimization of process parameters by multifactorial (i.e. DoE) approaches
- In-process control analysis for media parameters such as glucose, lactate, ammonia, amino acids, and several ions
- Cell analysis by cell cytometry
- HPLC-based analytics for product and impurity quantification (RP, SEC, IEC)
- Scale-up of batch/fed-batch cultures up to 400 l and perfusion cultures up to 50 l
- Tailoring of culture media components for fed-batch and perfusion
- Evaluation of critical process parameters
Methods and hardware
- Cell cultivation systems: culture flasks, spinner flasks, shake flasks, single-use wave-bioreactor systems up to 30 l, multi- and single-use stirred-tank bioreactor systems from 2 to 400 l
- Cell retention systems: spin filter, alternate tangential flow (ATF) system, acoustic settler (BioSep), gravitational settler, and centrifuge
- Culturing strategies: batch, repeated batch, fed-batch, perfusion, repeated perfusion
- Cell lines such as CHO, BHK, HEK, hybridoma, HL60, human amniocyte cells (CAP), and insect cells (Hi-5 and SF9)
- Depth filtration for subsequent downstream applications
