Decoding single cells

We improve your research on low-input samples

© Fraunhofer ITEM, Ralf Mohr

Our commitment is to support clients in developing perfectly tailored solutions for single-cell or rare-cell analysis.

We offer our clients expert knowledge in next-generation sequencing and microarray technologies, in particular at the single-cell DNA and RNA levels. Our in-house developed workflows are optimized for the analysis of clinical low-input or single-cell samples, e.g. cancer cells isolated from body fluids, fine-needle aspirates and tissue specimens. However, all protocols are fully customizable to the desired applications and your sample conditions. Our workflow integrates quality-control assays for optimal sample selection, technical implementation, and bioinformatics evaluation. Constant updates of the project progress include quality-control data of samples, key laboratory parameters, and related explanations. After project completion, a detailed project report with bioinformatics analysis is provided.

Our Services and expertise

© Fraunhofer ITEM, Ralf Mohr

Optimized sample preparation strategies for single cells and subpopulations:
Single-cell whole-genome and whole-transcriptome amplification technologies (Ampli1TM WGA and WTA, developed by the Fraunhofer ITEM team in Regensburg and the University of Regensburg), superior multi-level quality control assays for WGA and WTA samples to identify high-quality samples suited for analysis by high-throughput technologies (developed by the Fraunhofer ITEM team in Regensburg)

High-throughput molecular analysis of single cells and subpopulations:
Application and development of targeted molecular assays for single-cell analysis (panel sequencing), enhanced protocols for single-cell whole-exome sequencing, single-cell whole-genome sequencing, pure tumor cell isolation from FFPE samples and whole-exome sequencing, single-cell RNA-Seq analysis

Bioinformatic evaluation of single-cell-derived data sets:
Selection of bioinformatics evaluation level (DNA-based applications (raw data, aligned reads, variant calling, annotation), RNA-based applications (raw data, aligned reads, expression values, gene ontology, isoform prediction)), detailed bioinformatics analysis report




Your benefits

Comprehensive consulting and support for high-dimensional molecular analysis of your specific cells of interest

Access to a broad range of methods, customized solutions, and validated test systems

Solutions for and consulting on interpretation of bioinformatics data

As a non-university research institution we can be your partner in applying for public funding

ITEM-R is DIN ISO 9001:2015 certified