Enrichment, isolation and molecular analysis of rare cells

We deliver your cell of interest

© Fraunhofer ITEM

Our commitment is to drive innovative therapeutic approaches by decoding the underlying mechanisms in complex diseases on a single-cell level.

We offer our clients our expertise in the analysis of rare cell populations from fluids, tissue biopsies, and paraffin-embedded specimens. Our focus is on solid cancers, e.g. the analysis of circulating tumor cells (CTCs) and disseminated cancer cells (DCCs), however, our technologies can be adjusted for different fields of interest, such as stem cells in neurodegenerative diseases or regenerative medicine. Our expertise ranges from the development and implementation of individual enrichment and staining strategies to the isolation of pure cell populations, down to a single target cell.  

Our services and expertise

© Fraunhofer ITEM, Ralf Mohr


Enrichment of rare cell populations:
Marker-dependent enrichment  (including FDA-approved CellSearch® technology for CTC detection), marker-independent enrichment (microfluidic and filtration-based technologies), fluorescence activated cell sorting, laser capture microdissection, application of established staining protocols or implementation of customized solutions for your target of interest

Isolation of pure single cells:
Single-cell micromanipulation, dielectric cell sorting of single cells (DEPArrayTM technology)

Molecular analysis of single cells and subpopulations:
Single-cell whole-genome and whole-transcriptome amplification technologies (Ampli1TM WGA and WTA, developed for Silicon Biosystems by Fraunhofer ITEM’s team in Regensburg and the University of Regensburg, Germany), superior quality control assays for WGA and WTA samples to identify high-quality samples suited for your intended downstream analyses (developed for Silicon Biosystems by Fraunhofer ITEM’s team in Regensburg, Germany), application and development of targeted molecular assays for single-cell analysis, solutions for high-throughput analysis of nucleic acids (microarray analysis, RNA-sequencing, whole-exome sequencing and whole-genome sequencing of single cells)

Accredited lab for single-cell diagnostics (in cooperation with the University of Regensburg):
Access to a sample biobank generated from single CTCs/DCCs of patients with different cancer types for biomarker research and target validation, correlation with clinical follow-up possible for many samples, perfect environment for translational research within clinical studies

Your benefits

Comprehensive consulting and support for the recovery of your specific cells of interest

Access to a broad range of methods, customized solutions, and validated test systems

Solutions for and consulting on downstream molecular analysis

Assay and target validation on existing single-cell samples from different clinical cohorts

As a non-university research institution we can be your partner in applying for public funding


ITEM-R is DIN ISO 9001:2015 certified