Fraunhofer Sputum Core Facility

• Wissenschaftliche Beratung zum Studiendesign
• Auswahl der Sputum-Biomarker
• Studienspezifisches Aufsetzen der Sputum-Untersuchungsmethode
• Erstellen eines studienspezifischen Labor-Handbuchs / SOPs
• Zentrale und dezentrale Workshops zur Einweisung teilnehmender Zentren
  
• Fortlaufende Qualitätskontrolle
• Datenbericht / Publikation
• Probenversand und -lagerung
• Zentralisierte Sputum-Analyse
  • Zelluläre Sputum-Zusammensetzung (einschließlich FACS)
  • Biomarker in der Sputum-Flüssigphase (ELISA, MSD)
  • Chipzytometrie
  • Genexpression
  • Metabolomik (BIOKRATES)

Pedersen F, Trinkmann F, Abdo M , Kirsten AM, Rabe KF, Watz H, Baraldo S, Saetta M, Hohlfeld JM, Holz O. Influence of Cell Quality on Inflammatory Biomarkers in COPD Sputum Supernatant. Int J Chron Obstruct Pulmon Dis. 2021;16:487-493, https://doi.org/10.2147/COPD.S284938

Kern AL, Biller H, Klimeš F, Voskrebenzev A, Gutberlet M, Renne J, Müller M, Holz O, Wacker F, Hohlfeld JM, Vogel-Claussen J. Noninvasive Monitoring of the Response of Human Lungs to Low-Dose Lipopolysaccharide Inhalation Challenge Using MRI: A Feasibility Study. J Magn Reson Imaging. 2020 Jun; 51 (6): 1669-1676. doi: 10.1002/jmri.27000. Epub 2019 Nov 15. PMID: 31729119

Pedersen F, Zissler UM, Watz H, Rabe FK, Hohlfeld JM, Holz O. Rating sputum cell quality in clinical trials for asthma and COPD treatment. Int. Journal of COPD, 2019

Holz O, Biller H, Mueller M, Kane K, Rosano M, Hanrahan J, Hava DL, Hohlfeld JM. Efficacy and safety of inhaled calcium lactate PUR118 in the ozone challenge model - a clinical trial. BMC Pharmacol Toxicol. 2015

Janssen O, Schaumann F, Holz O, Lavae-Mokhtari B, Welker L, Winkler C, Biller H, Krug N, Hohlfeld JM. Low-dose endotoxin inhalation in healthy volunteers—a challenge model for early clinical drug development. BMC Pulm Med. 2013

Röpcke S, Holz O, Lauer G, Müller M, Rittinghausen S, Ernst P, Lahu G, Elmlinger M, Krug N, Hohlfeld JM. Repeatability of and relationship between potential COPD biomarkers in bronchoalveolar lavage, bronchial biopsies, serum, and induced sputum. PLoS One. 2012; 7 (10)

Biller H, Holz O, Windt H, Koch W, Müller M, Jörres RA, Krug N, Hohlfeld JM. Breath profiles by electronic nose correlate with systemic markers but not ozone response. Respir Med. 2011

Holz O, Khalilieh S, Ludwig-Sengpiel A, Watz H, Stryszak P, Soni P, Tsai M, Sadeh J, Magnussen H. SCH527123, a novel CXCR2 antagonist, inhibits ozone-induced neutrophilia in healthy subjects. Eur Respir J. 2010

Holz O, Tal-Singer R, Kanniess F, Simpson KJ, Gibson A, Vessey RS, Janicki S, Magnussen H, Jörres RA, Richter K. Validation of the human ozone challenge model as a tool for assessing anti-inflammatory drugs in early development. J Clin Pharmacol. 2005 May; 45 (5): 498-503. PubMed PMID: 15831772

Holz O, Mücke M, Paasch K, Böhme S, Timm P, Richter K, Magnussen H, Jörres RA. Repeated ozone exposures enhance bronchial allergen responses in subjects with rhinitis or asthma. Clin Exp Allergy. 2002

Paggiaro PL, Chanez P, Holz O, Ind PW, Djukanović R, Maestrelli P, Sterk PJ. Sputum induction. Eur Respir J Suppl. 2002

Fraunhofer ITEM